Harmful Algal Bloom Testing

 

Harmful Algal Bloom

Harmful Algal Bloom in Lake Dora, FL

Cyanobacteria, also known as blue-green algae, are photosynthetic, organisms naturally found in water bodies. Blue-green algae can proliferate and ‘bloom’ very quickly in response to warm temperatures, sunlight and high levels of nutrients. Algal blooms can deplete the oxygen and block sunlight to other organisms, and some can produce powerful toxins that are capable of causing illness and even death to wildlife, livestock, pets and even people. In addition to producing toxins, these harmful algal blooms (HABs) can produce taste and odor issues in public water supplies. Recommended maximum levels of the most common of these algal toxins in drinking water and waters used for recreation are extremely low, making many of these compounds among the most deadly toxins known to man. These problems can be caused by several genera of algae, including Microcystis, Planktothrix, Dolichospermum (formerly Anabaena), and Cylindrospermopsis.EnviroScience is experienced in the analysis of algae/cyanobacteria populations and quantification of common algal toxins.

EnviroScience offers the following in-house services:

Algae Toxin Testing via QPCR: NO GENE = NO TOXIN

A variety of environmental factors may determine whether a harmful algal bloom (HAB) is producing toxin(s) at a given time and place, and a bloom that is not producing toxins one day may produce them the next. Genomic cyanobacteria screening via DNA-based Quantitative Polymerase Chain Reaction, or qPCR, helps determine the potential for cyanotoxin production. It is possible to have a gene present without cyanotoxin production; however if cyanotoxins are being produced, the gene must be present. The presence or absence of toxin-producing genes in an algae bloom can help determine whether further testing for algal toxins via ELISA or another method is warranted.

Harmful Algal Bloom Surface Sample

Harmful Algal Bloom Surface Sample

 

qPCR tests for the following toxin genes are available from EnviroScience:

  • Microcystin/Nodularin
  • Cylindrospermopsin
  • Saxitoxin

A test for Anatoxin is under development and is expected to be available soon.

In addition, qPCR can estimate the number of cyanobacteria present in a bloom using the 16S rRNA gene.  Run concurrently with the toxin DNA tests, this test reports the percent of the cyanobacterial bloom that is capable of toxin production.

Contact one of our HAB specialists at 800-940-4025 for more information on these tests.

Algae Toxin Testing via ELISA Method

ELISA tests (enzyme linked immunosorbent assay tests) are screening assays that detect specific groups of cyanotoxins. ELISAs have the ability to detect these toxins at extremely low levels in both surface water and drinking water. EnviroScience biologists provide rapid turnaround, with most samples being completed within 2 business days.

(including many toxins on the U.S. EPA Candidate Contaminant List 3 (CCL 3))

  • Microcystin-LR
  • Anatoxin-a
  • Saxitoxins
  • Cylindrospermopsin 

 

Algae Identification and Enumeration

Algae and Algal Toxin Sampling

Simple DIY Monitoring

Algal B

Simple DIY Monitoring Kit

  • DIY sampling kits with intuitive sampling instructions and prepaid shipping
  • Experts who create a customized monitoring plan based on test results and the client’s need
  • 24 hr. turnaround analysis available for cyanotoxin analysis
  • Long-term solutions developed by an experienced team of limnologists and aquatic biologists

Comprehensive Lake/Reservoir Management

Other analyses are available upon request.

 

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